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plasmid expressing tev protease  (Addgene inc)


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    Addgene inc plasmid expressing tev protease
    Plasmid Expressing Tev Protease, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid expressing tev protease/product/Addgene inc
    Average 93 stars, based on 10 article reviews
    plasmid expressing tev protease - by Bioz Stars, 2026-03
    93/100 stars

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    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; <t>+TEV</t> – 2nd Ni-NTA flowthrough collected after <t>TEV</t> <t>protease</t> cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
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    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Journal: STAR protocols

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.

    doi: 10.1016/j.xpro.2023.102351

    Figure Lengend Snippet: Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Article Snippet: Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid.

    Techniques: SDS Page, Sequencing, Western Blot, Negative Control, Positive Control

    Journal: STAR Protocols

    Article Title: Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

    doi: 10.1016/j.xpro.2021.100431

    Figure Lengend Snippet:

    Article Snippet: Plasmid pRK793 for expressing tobacco etch virus (TEV) protease , , Addgene.org plasmid # 8827.

    Techniques: Virus, Recombinant, Thermal Shift Assay, Plasmid Preparation, Expressing, Modification, Sequencing, Software, Microscopy, Spectrophotometry, Protein Purification, Affinity Column, Membrane